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Materials and Methods Introduction of the Metal Binding and Reducing Peptide Sequence into C-terminal Surface Exposed Loops of TMV Coat Protein Plasmid pSNC004 containing a full-length TMV U1 genome under the control of a T7 promoter and modified in its coat protein sequence at nucleotides 193198 and 208213 to contain NgoMIV and BstZ17I sites, respectively, was provided by Dr Sean Chapman (The James Hutton Institute, Invergowrie, Dundee, DD2 5DA, UK)

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A 2022 report by the New Hampshire Department of Health and Human Services, titled Alcohol Overdose and Mortality Data, found that alcohol-induced deaths reached 289 statewide, with a rate of 17.0 per 100,000, exceeding the national average of 13.5

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